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Delitto perfetto : ウィキペディア英語版
Delitto perfetto
''Delitto perfetto'' () is a genetic technique for ''in vivo'' site-directed mutagenesis in yeast. This name is the Italian term for "perfect murder", and it refers to the ability of the technique to create desired genetic changes without leaving any foreign DNA in the genome.
== Background ==
This technique was developed by a group at the National Institute of Environmental Health Sciences (NIEHS) composed of Michael A. Resnick, Francesca Storici (now at Georgia Institute of Technology), and L. Kevin Lewis (now at Southwest Texas State University). The method uses synthetic oligonucleotides in combination with the cellular process of homologous recombination. Consequently, it is well suited for genetic manipulation of yeast, which has highly efficient homologous recombination. The ''delitto perfetto'' approach has been used to produce single and multiple point mutations, gene truncations or insertions, and whole gene deletions (including essential genes).

抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
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